Biomedical Laboratory techniques REPLICA F - ASL RIETI

Immunometry principles and use of antibodies. Antigen-antibody reaction. Antigen purification: affinity chromatography Main techniques involving the use of antibodies useful in laboratory diagnosis such as: immunofluorescence and immunoblotting, ELISA. Erythrocyte typing techniques: determination of the AB0 phenotype HLA typing techniques: Cytotoxicity test and mixed leukocyte reaction Diagnostic techniques for autoimmune diseases

To tackle the laboratory techniques course the student must have the basics of Biology, Chemistry and Biochemistry

Antonozzi, Gulletta Fondamenti di medicina di laboratorio, Piccin ed 2025 ppt and handouts

DESCRIBE THE PRINCIPLES OF IMMUNOMETRY AND USE OF ANTIBODIES. UNDERSTAND THE CONCEPT OF ANTI-GAP ANTI-BODY REACTIONS. DESCRIBE THE MONOCLONAL ANTIBODIES AND SPECIFY THE MAIN USES FOR THEIR CLINICAL DETERMINATION.