PHARMACOLOGICAL ASSAYS

Course objectives

GENERAL EXPECTED LEARNING OUTCOMES The main aims of the course are to provide knowledge on experimental pharmacological techniques essential for drug development. SPECIFIC EXPECTED LEARNING OUTCOMES At the end of the course, students 1. Knowledge and understanding. Will know some experimental pharmacological techniques essential for pre-clinical and clinical drug development. 2. Applying knowledge and understanding. Will develop critical skills on the subjects of the course also thanks to the preparation of a short theoretical dissertation and visits to research centers . 3. Making judgements. Will have acquired autonomy of judgment thanks to the independent analysis of the bibliographic sources of the course before and after the lectures (flipped classroom). 4. Communication skills. Will improve his/her communication skills also thanks to the presentation of a dissertation and the final oral exam. 5. Learning skills. Will be familiar with the most modern bibliographic tools (databases, online texbooks of the platform ACCESS MEDICINE) which will allow her/him to stay updated on all frontier subjects in the field of pharmacology.

Channel 1
LUCIANO SASO Lecturers' profile

Program - Frequency - Exams

Course program
1. HISTORY OF PHARMACOLOGY 2. DRUG DEVELOPMENT: PRECLINICAL AND CLINICAL EXPERIMENTATION (PHASES 1-4): THE INFORMED CONSENT: FROM THE NURENBERG CODE TO THE DECLARATION OF HELSINKI. 3. ANTIINFLAMMATORY DRUGS (STEROIDAL AND NON-STEROIDAL) AND IMMUNOSUPPRESSIVE DRUGS; EXPERIMENTAL METHODS: ULTRAVIOLET LIGHT INDUCED ERYTHEMA; CARRAGEENAN INDUCED EDEMA, COTTON PELLET GRANULOMA, ADJUVANT ARTHRITIS, RANDALL AND SELITTO TEST, HOT PLATE, TAIL FLICK, BREWER'S YEAST INFLAMMATION, EVALUATION OF THE ULCEROGENIC ACTIVITY. 4. STATISTICAL METHODS IN PHARMACOLOGY: CONCEPT OF SIGNIFICANT DIFFERENCE; PARAMETRIC AND NON PARAMETRIC TESTS (STUDENT’s, ANOVA) (MANN-WHITNEY, KRUSKAL-WALLIS); ALPHA AND BETA ERRORS; 5. PURIFICATION OF PROTEINS OF PHARMACOLOGICAL INTEREST: SAMPLE PREPARATION (DIALYSIS, ULTRAFILTRATION, CENTRIFUGATION, ETC); N-TERMINAL SEQUENCING; CHROMATOGRAPHIC FRACTIONATION AND ANALYSIS OF THE FRACTIONS BY BIOLOGICAL ASSAYS; CONVENTIONAL and HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC): GEL PERMEATION, IONIC EXCHANGE, ADSORPTION and AFFINITY; PREPARATION OF POLYCLONAL AND MONOCLONAL ANTIBODIES; POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE) IMMUNO-BLOTTING and CONCANAVALIN-A-BLOTTING; ELISA; RADIOIMMUNOASSAYS. ( 6. GUIDANCE FOR THE PREPARATION OF A THEORETICAL DISSERTATION ON ONE OF THE SUBJECTS OF THE COURSE
Prerequisites
No specific prerequisites
Books
Material provided by the professor
Frequency
Compulsory attendance
Exam mode
Questions on the all programme of the course
Lesson mode
Lectures and practical trainings
LUCIANO SASO Lecturers' profile

Program - Frequency - Exams

Course program
1. HISTORY OF PHARMACOLOGY 2. DRUG DEVELOPMENT: PRECLINICAL AND CLINICAL EXPERIMENTATION (PHASES 1-4): THE INFORMED CONSENT: FROM THE NURENBERG CODE TO THE DECLARATION OF HELSINKI. 3. ANTIINFLAMMATORY DRUGS (STEROIDAL AND NON-STEROIDAL) AND IMMUNOSUPPRESSIVE DRUGS; EXPERIMENTAL METHODS: ULTRAVIOLET LIGHT INDUCED ERYTHEMA; CARRAGEENAN INDUCED EDEMA, COTTON PELLET GRANULOMA, ADJUVANT ARTHRITIS, RANDALL AND SELITTO TEST, HOT PLATE, TAIL FLICK, BREWER'S YEAST INFLAMMATION, EVALUATION OF THE ULCEROGENIC ACTIVITY. 4. STATISTICAL METHODS IN PHARMACOLOGY: CONCEPT OF SIGNIFICANT DIFFERENCE; PARAMETRIC AND NON PARAMETRIC TESTS (STUDENT’s, ANOVA) (MANN-WHITNEY, KRUSKAL-WALLIS); ALPHA AND BETA ERRORS; 5. PURIFICATION OF PROTEINS OF PHARMACOLOGICAL INTEREST: SAMPLE PREPARATION (DIALYSIS, ULTRAFILTRATION, CENTRIFUGATION, ETC); N-TERMINAL SEQUENCING; CHROMATOGRAPHIC FRACTIONATION AND ANALYSIS OF THE FRACTIONS BY BIOLOGICAL ASSAYS; CONVENTIONAL and HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC): GEL PERMEATION, IONIC EXCHANGE, ADSORPTION and AFFINITY; PREPARATION OF POLYCLONAL AND MONOCLONAL ANTIBODIES; POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE) IMMUNO-BLOTTING and CONCANAVALIN-A-BLOTTING; ELISA; RADIOIMMUNOASSAYS. ( 6. GUIDANCE FOR THE PREPARATION OF A THEORETICAL DISSERTATION ON ONE OF THE SUBJECTS OF THE COURSE
Prerequisites
No specific prerequisites
Books
Material provided by the professor
Frequency
Compulsory attendance
Exam mode
Questions on the all programme of the course
Lesson mode
Lectures and practical trainings
  • Lesson code10612261
  • Academic year2025/2026
  • CourseIndustrial pharmacy
  • CurriculumSingle curriculum
  • Year5th year
  • Semester1st semester
  • SSDBIO/14
  • CFU6