MASS SPECTROMETRY APPLIED TO BIOINORGANIC CHEMISTRY

Course objectives

General expected learning outcomes The objective of the course on Mass Spectrometry Applied to Bioinorganic Chemistry is to provide the tools to design, execute, and interpret the results of an experimental approach based on mass spectrometry for studying issues related to bioinorganic chemistry. This discipline draws on basic concepts from inorganic chemistry, biochemistry, and the relevant analytical technology, mass spectrometry. Specific expected learning outcomes 1. Knowledge and understanding The student will learn about the most recent analytical techniques based on mass spectrometry for identifying and characterizing biologically significant species containing transition metals, including metallopharmaceuticals and metalloenzymes. 2. Applying knowledge and understanding At the end of the course, the student will be familiar with the main mass analyzers used for studying metallic species and will be able to define the pros and cons of different ionization sources used for sampling metallopharmaceuticals and metalloproteins, selecting them based on the characteristics of the analytes of interest. The student will also be capable of interpreting complex mass spectra. 3. Making judgements The lectures will be interactive, with questions and prompts designed to stimulate attention, make connections, and encourage critical considerations based on concepts and tools already acquired through exercises and laboratory experiences. The goal is to rework the learned notions and material obtained from bibliographic research in international journals and specialist texts in a clear and synthetic manner. 4. Communication skills To this end, the course includes the development of a monographic theme by each student and the related oral presentation in the presence of colleagues and potentially other interested parties. 5. Learning skills The student will be able to study and deepen the topics covered in the lectures using the material provided by the lecturer on the e-learning platform (slides, scientific articles, and reviews) and consulting the recommended textbooks (some copies are available in the Department library).

Channel 1
DAVIDE CORINTI Lecturers' profile

Program - Frequency - Exams

Course program
Application of mass spectrometry techniques to study ionic species of bioinorganic interest. Mass analysis of metal complexes with biological molecules. Characterization by mass spectrometry of the interaction affinity and of the binding sites of metal ions with amino acids and nucleotides. Interpretation of mass spectra and collision-induced dissociation spectra for metal complexes.
Prerequisites
A basic knowledge of the main concepts learned during the courses of General and Inorganic Chemistry, Organic Chemistry, Biology and Biochemistry is recommended.
Books
1) W. Kaim, B. Schwederski, A. Klein. "Bioinorganic Chemistry: Inorganic Elements in the Chemistry of Life Second edition, Wiley 2) I. Bertini, H. G. Gray, E. I. Stiefel, J. S. Valentine. "Biological Inorganic Chemistry. Structure and Reactivity" University Science Books
Frequency
Attendance at the course is recommended.
Exam mode
The final exam consists in the preparation, presentation of a ppt document and discussion of a topic chosen by the student from recent scientific literature.
Lesson mode
The course includes: 1) practical laboratory lessons on the preparation of samples for mass spectrometry and on the mass analysis of biologically relevant metal complexes; 2) exercises about the interpretation of mass spectra and fragmentation spectra. The learning material (slides presented during classes and scientific articles discussed) is available on the e-learning page of the course: https://elearning.uniroma1.it/.
DAVIDE CORINTI Lecturers' profile

Program - Frequency - Exams

Course program
Application of mass spectrometry techniques to study ionic species of bioinorganic interest. Mass analysis of metal complexes with biological molecules. Characterization by mass spectrometry of the interaction affinity and of the binding sites of metal ions with amino acids and nucleotides. Interpretation of mass spectra and collision-induced dissociation spectra for metal complexes.
Prerequisites
A basic knowledge of the main concepts learned during the courses of General and Inorganic Chemistry, Organic Chemistry, Biology and Biochemistry is recommended.
Books
1) W. Kaim, B. Schwederski, A. Klein. "Bioinorganic Chemistry: Inorganic Elements in the Chemistry of Life Second edition, Wiley 2) I. Bertini, H. G. Gray, E. I. Stiefel, J. S. Valentine. "Biological Inorganic Chemistry. Structure and Reactivity" University Science Books
Frequency
Attendance at the course is recommended.
Exam mode
The final exam consists in the preparation, presentation of a ppt document and discussion of a topic chosen by the student from recent scientific literature.
Lesson mode
The course includes: 1) practical laboratory lessons on the preparation of samples for mass spectrometry and on the mass analysis of biologically relevant metal complexes; 2) exercises about the interpretation of mass spectra and fragmentation spectra. The learning material (slides presented during classes and scientific articles discussed) is available on the e-learning page of the course: https://elearning.uniroma1.it/.
  • Lesson codeAAF2374
  • Academic year2025/2026
  • CourseIndustrial pharmacy
  • CurriculumSingle curriculum
  • Year5th year
  • Semester1st semester
  • CFU2