Medicine and Surgery

General General principles and applications of clinical biochemistry and clinical molecular biology. Description and use of the main clinical biochemistry laboratory tests and their use in the corresponding diagnostic and therapeutic pathways. Description and use of the main clinical molecular biology laboratory tests and examples of use in the diagnosis of the most frequent genetic diseases. Specific subjects Appropriateness in Laboratory Medicine. Correct diagnostic procedure. Knowing how to request a laboratory investigation. Analytical and biological variability. Sample collection. Precision, accuracy, analytical sensitivity and specificity. Errors in Laboratory Medicine. The intra and inter laboratory quality checks. Knowing how to interpret a laboratory survey. Reference intervals, critical values and decision limits. Critical difference. How to evaluate a diagnostic test. Prevalence, diagnostic sensitivity and specificity. Diagnostic efficacy and positive and negative predictive values. ROC Curves. Main analytical methods of biochemistry: spectrophotometry, spectrofluorimetry, nephelometry, electrophoresis. Main analytical methods of molecular biology: in vitro enzymatic amplification (PCR), direct hybridization, reverse hybridization, PCR/OLA/SCS, DNA sequencing, principles of next generation sequencing. Functions of plasmaproteins. Electrophoretic separation of serum proteins. The electrophoretic path (description of the main proteins separated electrophoretically and clinical indications to their request). The electrophoresis report. Conditions associated with specific alterations of the electrophoretic pattern. Poly and monoclonal gammopathies and their characterization. Immunofixation and immunosubctraction. False monoclonal components. Laboratory quality control. General principles and applications of clinical molecular biology.

Basic knowledge of mathematics, physics, chemistry, biology, biochemistry and physiology.

Ciaccio. Trattato di Biochimica Clinica e Medicina di Laboratorio. Edizioni Edises. None in English.

Theoretical and applicative frontal lessons; theoretical and practical elective and integrative activities.

The attendance to theoretical and applicative frontal lessons and to trainings are mandatory. The attendance is checked by the teacher by using updated lists provided by the Academic Office that will be signed by the students. The certificate of attendance of the teaching course is required for the student to be admitted to the final test.

To Pass the test student must obtain a note of 18/30 for each subject of the integrated teach. Student must possess a sufficient knowledge of the program To obtain a note of 30/30 with distinction student must possess an excellent knowledge of the whole program using a correct terminology to expose the topics

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Theoretical and applicative frontal lessons; theoretical and practical elective and integrative activities. The teaching is based essentially on frontal lessons characterized by a strong interactive component between the teacher and the students. Other teaching methods may include working groups or individual activities focused on the analysis of methods and results of publications in international scientific journals. There are practical sessions and exercises.