GENERAL PATHOLOGY AND PATHOPHYSIOLOGY II - Technical Sciences of Laboratory Medicine

Course objectives

The integrated course of Pathology and Pathophysiology and technical sciences of laboratory medicine aims to transmit the notions concerning the mechanisms that cause diseases and the molecular mechanisms that underlie all organ pathology and any systemic pathology. It also concerns the interpretation of the molecular and cellular mechanisms that underlie the different pathologies. In this regard, students will have to know the basis of the advanced methods of identification of molecular mechanisms and therefore their translational application in the pathology. Students, following completion of the course, must be able to recognize and interpret the pathogenic mechanisms and causes of diseases. They will also be able to correlate the molecular aspects of damage and pathology with the signs and symptoms of the disease and then with the clinical manifestations of each pathology, also indicating which clinical-molecular examinations are indicated in different pathologies.

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ISOTTA CHIMENTI Lecturers' profile

Program - Frequency - Exams

Course program
Professionalizing activities in small groups to be carried out in the research laboratories of the Department of Medical-Surgical Sciences and Biotechnology - Corso della Repubblica 79. Students will be divided into groups of 7-8 people, each with a contact person responsible for communicating with the teacher responsible for the activities, for a total of 10 groups per semester, which will rotate every two weeks following an annual calendar published on the E-learning platform at the beginning of the relevant academic year. The activities will be aimed at acquiring the following skills: - Carrying out cellular stress tests with evaluation of the result - Carrying out chemosensitivity tests on tumor cells and in vitro evaluation Students will initially be introduced to laboratory safety and biohazard containment. The main topics covered within the professionalizing activities concern the effects of oxidative stress and/or chemotherapy on tumor cells. The basic principles of cell cultures and the possible responses to cellular stress and/or pharmacological resistance will then be illustrated (e.g. cell viability tests, quantification of necrosis, apoptosis or autophagy, pro-fibrotic or pro-inflammatory activation). In particular, a classic experiment is designed and set up together with the students which involves: use of the optical microscope for the observation of pre- and post-treatment cell cultures, morphological evaluation, cell counting using Trypan Blue assay, graph of the results obtained with consequent construction of a dose-response curve. At the end of the activities, the responsible teacher discusses the results obtained with the students, contextualizing them with the scientific literature. Students then learn to search for scientific articles and compare their findings with the most recent literature.
Books
Patologia generale e Fisiopatologia generale VI Edizione G.M. Pontieri Piccin Editore Educational material provided during the course (including scientific papers available on PubMed platform) and uploaded as PDF files on the E-learning platform.
PAOLO ROSA Lecturers' profile
  • Academic year2025/2026
  • CourseMedicine and Surgery
  • CurriculumSingle curriculum
  • Year3rd year
  • Semester1st semester
  • SSDMED/46
  • CFU2