THREE-DIMENSIONAL MODELING

Course objectives

General skills Today’s view of Microbiology has gone far beyond the simple idea of microbes as causative agents of a diversified spectrum of human pathologies. The current opinion considers microorganisms mainly as vital components of eukaryotic organisms, major constituents of ecosystems, and key mediators in productive and industrial processes. Our course in Microbiology and Virology is therefore intended to provide students with the knowledge clues required to understand the dual nature of the microbial world. The focus is on progressively integrating specific notions about bacterial and viral structures, their functional relationships, and the organization and evolution of genomes into a logical learning path. This will secure the understanding of the molecular mechanisms which regulate the interaction of microorganisms with both biotic and abiotic environments. Students will thus acquire the competences to assess the role of microorganisms in biomedical, biotechnological, and environmental area. Specific skills A) Knowledge and understanding. The student will have acquired knowledge and understanding - Structure of the prokaryotic cell (Bacteria and Archea) and biosynthesis of the cell components. - Organization of the bacterial genome and role of the mobile genetic elements - Procedures for microbial growth and for microbial growth control - Processes of bacterial colonization of the host and of the environment - Mechanisms of action of antibiotics and emergence of antibiotic resistance B) Applying knowledge and understanding - Understanding the key role played by microrganisms in the interactions with the host and the environment. - Understanding the biological processes of multicellular organisms banking on the knowledge acquired from the microrganisms C) Making judgments - Being able to analyze in a thoughtful and informed manner the information on the microbes world - Making informed decisions on the use of products derived from bacteria and on the importance of vaccinations D) Communication skills - Being able to communicate to the public the notions learned on the microbe world in a correct and informed manner - Being able to fully interpret any microorganisms-associated phenomena from both a personal and social point of view. E) Learning skills -be able to make logical connection among different topics covered -learning the specific terminology

Channel 1
MARTINA PASQUA Lecturers' profile

Program - Frequency - Exams

Prerequisites
The Course of Microbiology and Virology takes place at the third year in the first semester. For the full understanding of the topics of the Course a sound knowledge of Cellular Biology, Genetics, Molecular Biology and Biochemistry is necessary
Books
Part1. Microbiology Dehò G., Galli E. Biologia dei Microrganismi . 2014. Casa Editrice Ambrosiana (CEA) Brock Biologia dei Microrganismi .1. Microbiologia generale. 2012 Pearson Ed. Maresca M. Microbiologia molecolare e cellulare 2013. McGrraw-Hill Ed. A PDF copy of the lessons and the programme of the exam are available at the elearning platform ( Micorbiology part site).
Frequency
Class attendance is not mandatory. To participate to writen evaluation test held at the end of frontal lessons students must have attended the majority of the lessons
Exam mode
The examination aims to verify the level of knowledge of the topics and as well as the reasoning skills acquired by the student. The evaluation is expressed in thirtieths (minimum score 18/30, maximum score 30/30 with honors) and corresponds to the weighted average of the scores obtained in the two parts of the Course. The evaluation consists of an oral examination for the Microbiology Part and an oral examination for the Virology Part. The examination is carried out taking into consideration the different topics covered during the course and allows to verify the achievement of the objectives in terms of knowledge and skills acquired, to evaluate, the language property and the critical capacity of the student. To pass the examination it is necessary to obtain at least the score of 18/30 in both Microbiology and Virology part. The final score is determined by the weighted average of the score obtained in each part. To achieve the score of 30/30 cum laude, the student must demonstrate to have acquired an excellent knowledge of the topics covered in the two parts of the Course and to be able to create logical and coherent connections between the various topics studied.
Lesson mode
The course is structured in theoretical lectures (5.0 CFU ) and a Laboratory session ( 1 CFU) Classes are held three times a week in the classroom. The laboratory session (12 h) is organized in groups of 20 people (with appropriate turnaround) and takes place in laboratories equipped for experiments in Microbiology and Virology.
BIANCA COLONNA Lecturers' profile
Channel 2
GIANNI PROSSEDA Lecturers' profile

Program - Frequency - Exams

Course program
Theoretical Lectures Part 1: MICROBIOLOGY (40 hours) Structure and function of the prokaryotic cell (20 hours). The historical roots of microbiology. Physiological diversity of microrganisms. Structure, function and biosynthesis of the cell wall in Bacteria and Archea. Secretion and exportation in Bacteria. Function and structure of the cytoplasmic membrane. Biosynthesis and function of cell surface structures. Bacterial cell division and sporulation. Microbial nutrition and culture media. Regulation of bacterial gene expression. Bacterial genome, plasticity and evolution (10 hours) Organization of bacterial chromosome: the nucleoid. Structure and function of plasmids. The main features of transposable elements and integrons. The role of mobile genetic elements in the horizontal gene transfer. Bacterial conjugation and transformation. Interaction of the microrganisms with biotic and abiotic environments (10 hours) Overview of human microbiome and microbiota. Mechanisms of actions of toxins. Virulence factors and host defence mechanisms. Quorum sensing and biofilms. Mechanisms of action of antibiotics and antibiotic resistance. Signal transductions: from porin biosynthesis to chemiotaxis. Beneficial and harmful microbial interactions with eukaryotic hosts. Bacterial adaptation to the host response. Principles of innate immunity. Laboratory session (12 hours) Preparation of Bacterial culture media. Measurement of bacterial growth: dilutions and viable counts. The Gram stain procedure and the microscopic observation of bacterial cells. Antibiotic susceptibility tests. Ames test: bacterial response to UV radiation. Procedures for quantification of viruses. Analysis of bacteriophages: lysis and plaque assay.
Prerequisites
The Course of Microbiology and Virology takes place at the third year in the first semester. For the full understanding of the topics of the Course a sound knowledge of Cellular Biology, Genetics, Molecular Biology and Biochemistry is necessary
Books
Gianni Dehò e Enrica Galli "Biologia dei microrganismi" seconda edizione Madigan M.T. and Martinko J.M. : Brock, Biologia dei microorganismi.
Teaching mode
The course is structured in lectures and didactic laboratories. In particular, 40 hours of frontal teaching (5 CFU) and 12 hours of didactic laboratories (1 CFU) are provided. Lessons take place three times a week in the classroom. Attendance at lessons is strongly recommended. The didactic laboratories are carried out in groups of 20-25 people (with specific shifts) and take place in laboratories equipped for the teaching of Microbiology and Virology.
Frequency
Although the attendance at lessons and didactic laboratories is not mandatory, it is strongly recommended
Exam mode
The examination aims to verify the level of knowledge of the topics and as well as the reasoning skills acquired by the student. The evaluation is expressed in thirtieths (minimum score 18/30, maximum score 30/30 with honors) and corresponds to the weighted average of the scores obtained in the two parts of the Course. The evaluation consists of an oral examination for the Microbiology Part and an oral examination for the Virology Part. The examination is carried out taking into consideration the different topics covered during the course and allows to verify the achievement of the objectives in terms of knowledge and skills acquired, to evaluate, the language property and the critical capacity of the student. To pass the examination it is necessary to obtain at least the score of 18/30 in both Microbiology and Virology part. The final score is determined by the weighted average of the score obtained in each part. To achieve the score of 30/30 cum laude, the student must demonstrate to have acquired an excellent knowledge of the topics covered in the two parts of the Course and to be able to create logical and coherent connections between the various topics studied.
Lesson mode
The course is structured in lectures and didactic laboratories. In particular, 40 hours of frontal teaching (5 CFU) and 12 hours of didactic laboratories (1 CFU) are provided. Lessons take place three times a week in the classroom. Attendance at lessons is strongly recommended. The didactic laboratories are carried out in groups of 20-25 people (with specific shifts) and take place in laboratories equipped for the teaching of Microbiology and Virology.
NADIA ANDREA ANDREANI Lecturers' profile

Program - Frequency - Exams

Course program
The teaching workshop will cover the following topics: - phage transduction - purifies on selective and indicator media - antibiogram
Prerequisites
In order to profitably follow the didactic laboratory of the MICROBIOLOGY AND VIROLOGY MICROBIOLOGY 2 course, it is necessary for the student to have followed and understood the topics covered in the theoretical lectures of the course itself.
Books
Texts Recommended for all modules: Madigan M.T. and Martinko J.M. : Brock, Biology of Microorganisms. Volume 1 (for Prof. Prosseda-Valenti module ) and 2b (Valenti module). Optional: Snyder L. and Champness W. Molecular Genetics of Bacteria. Department of Biology and Biotechnology “C. Darwin” Library.
Frequency
Although attendance is not mandatory, attending classes and workshops is strongly recommended.
Exam mode
Evaluation will be done as part of the evaluation of the theory part.
Lesson mode
Class attendance is strongly recommended. There are 12 hours of didactic workshops (1 CFU). Classes are held on a three-weekly basis in the classroom. Didactic labs are conducted in groups of 20-25 people (with appropriate rotation) and are held in laboratories equipped for teaching Microbiology and Virology after passing 60 percent of the theoretical lectures.
  • Academic year2025/2026
  • CourseBiology
  • CurriculumBiosanitario
  • Year3rd year
  • Semester1st semester
  • SSDBIO/19
  • CFU6