BIOLOGY OF DEVELOPMENT

Course objectives

General skills The course is focused on the study of embryology and the mechanisms that control the different phases of embryogenesis and cell differentiation and on the gametogenesis and fertilization processes. The main aims of the course is to acquire the basic knowledge of the various phases of embryogenesis with a comparative view on different animal models (invertebrates and vertebrates) and of the main mechanisms that control cell fate and specification/differentiation, the maturation of gametes and the mechanisms of fertilization. The course requires established knowledge of cell biology and the basic mechanisms of molecular biology. The course includes lessons and laboratory sessions, dedicated to the observation and recognition of embryos of different animal models and the observation of histological sections of embryos at various developmental stages. The student should have basic knowledge of the microscope and the main histological techniques. The course includes at the end 2-3 bio-applicative seminars with the aim to discuss topics such as animal cloning, the use of adult stem cells and iPS in regenerative medicine, assisted fertilization Specific skills Knowledge and understanding - Knowledge of the stages of embryogenesis and of the main events characterizing the cell fate and differentiation -Knowledge and understanding of the various stages of embryo development with a comparative vision -Knowledge and understanding of the mechanisms that control the cell fate - Knowledge and understanding of the germ line formation and the gametes maturation. B) Applying knowledge and understanding -know how to use the appropriate terminology - recognize the various phases of embryogenesis - recognize the various embryos and the main tissues and organs in development both in whole embryos and in histological sections C)Autonomy of judgment - acquiring critical judgment skills, through the historical study of the developmental biology knowledge and of the evolution of the different methodologies used starting from experimental embryology to the recent molecular biology - evaluation of the knowledge learned through self-assessment tests and through an exception test D) Communication skills -Describe what has been learned through the oral test using scientific language properties and knowing how to integrate, discuss and analyze in a critical way what has been learned E) Learning skills - learn the appropriate terminology - connect and integrate the acquired knowledge in a logical way - identify the most relevant topics and their potential applicability to current problems.

Channel 1
GIUSEPPE LUPO Lecturers' profile

Program - Frequency - Exams

Course program
History of the embriology and of the developmental biology. Morphological, experimental and molecular approaches to study the developmental biology. Embryonic and post-embryonic development. Reproductive modalities. Gametogenesis. Fertilization. Developmental stages. Description of the development of different model systems: Sea Urchin, Drosophila, Anfioxus, Zebrafish, Amphibia, Chick, and Mammalian. Germ layer derivatives and outlines of organogenesis. Genomic equivalence. Cell fate determination. Autonomous or conditioned specification. Cell adhesion and morphogenesis. Example of cell commitment and differentiation: miogenesis and neural crest formation. Axis pattern formation in Drosophila and Vertebrates. Primary and secondary induction. Limb development. LABORATORY SESSIONS (12 hrs). Analysis and observation of fresh and fixed embryos of Sea Urchin, Amphibia, Chick and Drosphila. In vitro fertilization using Sea Urchin gametes. Observation at light microscope of sections of embryos at different developmental stages and projection of short movies. Histological analysis of sections of rat gonads.
Prerequisites
Knowledge of cell biology and histology as taught during the first term of the first year of the Biological Sciences course.
Books
Choice 1. S. Gilbert. Developmental Biology. V edizione. Ed. Zanichelli 2. Wolpert. Developmental Biology. II edizione, Ed. Zanichelli Additional material is made available by the teacher on the e-learning platform2: https://elearning2.uniroma1.it/course/view.php?id=1932
Teaching mode
The course includes lectures with the use of power point slides. Lectures include 64h in the classroom (= 32 lectures 2h each). The laboratory activity is carried out in small groups. Students have microscope and stereomicroscope for observing the embryo preparations. Through the lectures the students learn the fundamental knowledge of the discipline. The laboratories are aimed at completing and applying the knowledge acquired in the classroom.
Frequency
Lectures are not mandatory. At least 80% of laboratory sessions are mandatory.
Exam mode
The final exam test aims to verify the level of knowledge and in-depth analysis of the topics covered and the critical analysis developed by the student. The evaluation is expressed in thirtieths (minimum grade 18/30, maximum mark 30/30 cum laude). The assessment consists of an oral exam preceded by an exoneration test carried out in itinere (evaluated by vote) or by an initial written test (aimed at assessing the admission of the student to the oral examination) based on images and schemes that must be recognized and commented by the students. In the oral exam the property of language, the clarity of exposition and the critical and integration capacity between the various topics are evaluated. The final grade results from the average between the written exoneration test and the oral exam. Both tests are passed with a minimum evaluation of 18/30.
Bibliography
1. S. Gilbert. Biologia dello Sviluppo. V edizione. Ed. Zanichelli 2. Wolpert. Biologia dello Sviluppo. II edizione. Ed. Zanichelli 3. Menegola, Bonfanti, Colombo, Del Giacco, Manuale di Biologia dello Sviluppo Animale, EdiSES (2019) 4. Barbieri, Carinci, Embriologia, Casa Editrice Ambrosiana, terza edizione (2015) (CHAPTERS ON SPERMATOGENESIS AND OVOGENESIS)
Lesson mode
The course includes lectures with the use of power point slides. Lectures include 64h in the classroom (= 32 lectures 2h each). The laboratory activity is carried out in small groups. Students have microscope and stereomicroscope for observing the embryo preparations. Through the lectures the students learn the fundamental knowledge of the discipline. The laboratories are aimed at completing and applying the knowledge acquired in the classroom.
GIUSEPPE LUPO Lecturers' profile

Program - Frequency - Exams

Course program
History of the embriology and of the developmental biology. Morphological, experimental and molecular approaches to study the developmental biology. Embryonic and post-embryonic development. Reproductive modalities. Gametogenesis. Fertilization. Developmental stages. Description of the development of different model systems: Sea Urchin, Drosophila, Anfioxus, Zebrafish, Amphibia, Chick, and Mammalian. Germ layer derivatives and outlines of organogenesis. Genomic equivalence. Cell fate determination. Autonomous or conditioned specification. Cell adhesion and morphogenesis. Example of cell commitment and differentiation: miogenesis and neural crest formation. Axis pattern formation in Drosophila and Vertebrates. Primary and secondary induction. Limb development. LABORATORY SESSIONS (12 hrs). Analysis and observation of fresh and fixed embryos of Sea Urchin, Amphibia, Chick and Drosphila. In vitro fertilization using Sea Urchin gametes. Observation at light microscope of sections of embryos at different developmental stages and projection of short movies. Histological analysis of sections of rat gonads.
Prerequisites
Knowledge of cell biology and histology as taught during the first term of the first year of the Biological Sciences course.
Books
Choice 1. S. Gilbert. Developmental Biology. V edizione. Ed. Zanichelli 2. Wolpert. Developmental Biology. II edizione, Ed. Zanichelli Additional material is made available by the teacher on the e-learning platform2: https://elearning2.uniroma1.it/course/view.php?id=1932
Teaching mode
The course includes lectures with the use of power point slides. Lectures include 64h in the classroom (= 32 lectures 2h each). The laboratory activity is carried out in small groups. Students have microscope and stereomicroscope for observing the embryo preparations. Through the lectures the students learn the fundamental knowledge of the discipline. The laboratories are aimed at completing and applying the knowledge acquired in the classroom.
Frequency
Lectures are not mandatory. At least 80% of laboratory sessions are mandatory.
Exam mode
The final exam test aims to verify the level of knowledge and in-depth analysis of the topics covered and the critical analysis developed by the student. The evaluation is expressed in thirtieths (minimum grade 18/30, maximum mark 30/30 cum laude). The assessment consists of an oral exam preceded by an exoneration test carried out in itinere (evaluated by vote) or by an initial written test (aimed at assessing the admission of the student to the oral examination) based on images and schemes that must be recognized and commented by the students. In the oral exam the property of language, the clarity of exposition and the critical and integration capacity between the various topics are evaluated. The final grade results from the average between the written exoneration test and the oral exam. Both tests are passed with a minimum evaluation of 18/30.
Bibliography
1. S. Gilbert. Biologia dello Sviluppo. V edizione. Ed. Zanichelli 2. Wolpert. Biologia dello Sviluppo. II edizione. Ed. Zanichelli 3. Menegola, Bonfanti, Colombo, Del Giacco, Manuale di Biologia dello Sviluppo Animale, EdiSES (2019) 4. Barbieri, Carinci, Embriologia, Casa Editrice Ambrosiana, terza edizione (2015) (CHAPTERS ON SPERMATOGENESIS AND OVOGENESIS)
Lesson mode
The course includes lectures with the use of power point slides. Lectures include 64h in the classroom (= 32 lectures 2h each). The laboratory activity is carried out in small groups. Students have microscope and stereomicroscope for observing the embryo preparations. Through the lectures the students learn the fundamental knowledge of the discipline. The laboratories are aimed at completing and applying the knowledge acquired in the classroom.
Channel 2
ADA MARIA TATA Lecturers' profile

Program - Frequency - Exams

Course program
History of the embriology and of the developmental biology. Morphological, experimental and genetic approaches to study the developmental biology. Embryonic and post-embryonic development. Reproductive modalities. Gametogenesis. Fertilization. Developmental stages. Description of the development of different model systems: Sea Urchin, Drosophila, Amphibia, Zebrafish, Xenopus, Chick, and Mammalian. Germ layer derivatives and outlines of organogenesis. Genomic equivalence. Cell fate determination. Autonomous or conditioned specification. Cell adhesion and morphogenesis. Example of cell commitment and differentiation: miogenesis and neural crest formation. Axis pattern formation in Drosophila and Vertebrates. Primary and secondary induction. Limb development. Biotechnological perspectives: therapeutic clonation, stem cells. Embryonic and adult stem cells: origin, potentiality and limits. Biological implication of the In vitro fertilization. LABORATORY SESSIONS (12 hrs). Analysis and observation of fresh and fixed embryos of Sea Urchin, Amphibia, Chick and Drosphila. In vitro fertilization using Sea Urchin gametes. Observation at light microscope of sections of embryos at different developmental stages and projection of short movies. Histological analysis of sections of rat gonads.
Prerequisites
Blended modality
Books
Menegola, Bonfanti, Colombo, del Giacco. Manuale di biologia dello sviluppo animale. Ed. Edises. (for the embriology) Choise between 1.S. Gilbert. Developmental Biology. IV edizione. Ed. Zanichelli 2. Wolpert. Developmental Biology. Ed. Zanichelli 3. Giudice, Campanella, Augusti Tocco. Developmental Biology. Ed. Piccin Additional material is made available by the teacher on the e-learning platform2: https://elearning2.uniroma1.it
Teaching mode
lessons in classroom through power point presentation. Blended considering the possible pandemic restriction.
Frequency
In classroom
Exam mode
The exam will take place by writenn test follewed by oral exam. A written test on the first part of the program will be performed previously. Ongoing tests are scheduled
Lesson mode
lessons in classroom through power point presentation. Blended considering the possible pandemic restriction.
ADA MARIA TATA Lecturers' profile

Program - Frequency - Exams

Course program
History of the embriology and of the developmental biology. Morphological, experimental and genetic approaches to study the developmental biology. Embryonic and post-embryonic development. Reproductive modalities. Gametogenesis. Fertilization. Developmental stages. Description of the development of different model systems: Sea Urchin, Drosophila, Amphibia, Zebrafish, Xenopus, Chick, and Mammalian. Germ layer derivatives and outlines of organogenesis. Genomic equivalence. Cell fate determination. Autonomous or conditioned specification. Cell adhesion and morphogenesis. Example of cell commitment and differentiation: miogenesis and neural crest formation. Axis pattern formation in Drosophila and Vertebrates. Primary and secondary induction. Limb development. Biotechnological perspectives: therapeutic clonation, stem cells. Embryonic and adult stem cells: origin, potentiality and limits. Biological implication of the In vitro fertilization. LABORATORY SESSIONS (12 hrs). Analysis and observation of fresh and fixed embryos of Sea Urchin, Amphibia, Chick and Drosphila. In vitro fertilization using Sea Urchin gametes. Observation at light microscope of sections of embryos at different developmental stages and projection of short movies. Histological analysis of sections of rat gonads.
Prerequisites
Blended modality
Books
Menegola, Bonfanti, Colombo, del Giacco. Manuale di biologia dello sviluppo animale. Ed. Edises. (for the embriology) Choise between 1.S. Gilbert. Developmental Biology. IV edizione. Ed. Zanichelli 2. Wolpert. Developmental Biology. Ed. Zanichelli 3. Giudice, Campanella, Augusti Tocco. Developmental Biology. Ed. Piccin Additional material is made available by the teacher on the e-learning platform2: https://elearning2.uniroma1.it
Teaching mode
lessons in classroom through power point presentation. Blended considering the possible pandemic restriction.
Frequency
In classroom
Exam mode
The exam will take place by writenn test follewed by oral exam. A written test on the first part of the program will be performed previously. Ongoing tests are scheduled
Lesson mode
lessons in classroom through power point presentation. Blended considering the possible pandemic restriction.
  • Lesson code1019202
  • Academic year2024/2025
  • CourseBiology
  • CurriculumBiotecnologico cellulare
  • Year2nd year
  • Semester2nd semester
  • SSDBIO/06
  • CFU9
  • Subject areaDiscipline botaniche, zoologiche, ecologiche