BIOCHEMICAL CLINICAL ANALYSIS

Course objectives

General skills The general purpose of the course is to learn the principles and aims of clinical biochemistry. The most frequently used analytical techniques in biochemical analysis laboratories are described. The main objectives are the learning of the principles and purposes of biochemistry laboratory, the development of the critical interpretation skills of the results of laboratory tests and the critical evaluation of the various analytical procedures. The program includes the presentation of some of the main methods of clinical laboratory investigation and their application in the diagnosis of diseases. The course requires basic knowledge of chemistry and biochemistry acquired in the courses of the first years of the three-year degree in Biological Sciences. Specific skills A) Knowledge and understanding - Knowing and understanding the principles of biochemistry techniques: Centrifugation, Chromatography, Electrophoresis, Spectroscophy - Immunological techniques and their diagnostic applications - Determinations of enzymatic activities - Diagnosis of some pathologies B) Applying knowledge and understanding - Be able to use the specific terminology - Knowing how to identify the right procedures to solve some questions of Clinical Biochemistry C) Making judgements - Acquire analytical skills, also through the study of the progress of knowledge in Clinical Biochemistry - Ask questions for the elaboration and deepening of the knowledge learned D) Communication skills - Be able to communicate what has been learned during the oral exam E) Learning skills - Learning the specific terminology - Be able to make the logical connections between the topics covered

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EMANUELE CACCI Lecturers' profile

Program - Frequency - Exams

Course program
- Technical characteristics of the clinical biochemistry laboratory tests. Principles of interpretation of laboratory tests. Reliability of clinical biochemistry analyses. - Collection and fractionation of biological materials. Centrifugation techniques. Chromatographic techniques. - Electrophoretic techniques. Single and two-dimensional electrophoresis. Isoelectric focusing. Electrophoretic patterns of biological liquids and their diagnostic use (serum proteins, hemoglobins, urinary proteins). - Spectroscopic Techniques. Ultraviolet and visible spectroscopy. Spectrofluorimetry. Turbidimetry and nephelometry. Luminometry. - Determinations of enzymatic activities. Enzymatic and isoenzymatic patterns of tissues and body fluids. Dosages of metabolites, evaluation of the metabolic states. Immobilized enzymes. Biosensors. - Production and use of antisera and monoclonal antibodies. Immunological techniques and their diagnostic applications (immunoprecipitation, immunoelectrophoresis tests, immunoenzymatic and radioimmunoassay methods, immunoblotting, immunofluorescence). - Blood groups and transfusion compatibility tests.
Prerequisites
Knowledge of the basic concepts of Biochemistry, immunology and physiology.
Frequency
Attendance is not mandatory although required for participation in the laboratory activities scheduled at the end of the course
Exam mode
An oral interview will be used to assess student learning and knowledge
Lesson mode
The course is based on 40 hours of face-to-face teaching (two hours/lectures). Lessons will be held in classroom through the help of power-point presentations prepared by the teacher and movies downloaded from international electronic scientific journals. Movies will be used to show how the main experimental procedures, discussed during the course, are performed in the laboratory
  • Lesson code1019007
  • Academic year2024/2025
  • CourseBiology
  • CurriculumBiosanitario
  • Year3rd year
  • Semester1st semester
  • SSDBIO/12
  • CFU6
  • Subject areaAttività formative affini o integrative