ANCIENT DNA AND METABARCODING

Course objectives

Second module: Students will be able to reconstruct past cultural landscapesand the impact of human populations on the environment, through advanced techniques. A - Knowledge and understanding OF 1) Provide students with a solid theoretical and methodological foundation for applying biological sciences to the study and preservation of cultural heritage. OF 2) Identify main biological agents responsible for the biodeterioration of artworks, and understand mechanisms of action to develop appropriate conservation and prevention strategies. OF 3) Gain familiarity with biological analysis techniques, from traditional methods to advanced approaches such as metabarcoding and metagenomics, applied to both modern and ancient DNA. OF 4) Understand advanced methods used to study biodeterioration with a focus on the impact of human activities on cultural heritage. OF 5) Deepen knowledge of genetic diversity, molecular markers, and bioinformatic tools for the identification of organisms involved in deterioration processes. … B - Application skills OF 7) Apply DNA analysis methods to cultural heritage, with a specific focus on diagnostics and the conservation of artworks. OF 8) Perform basic laboratory techniques, such as DNA extraction, PCR amplification, DNA fingerprinting and sequencing. OF 9) Develop practical skills in laboratory work, write short scientific reports, and present research findings in the form of seminars. … C - Autonomy of judgment OF 10) Assess the extent of biodeterioration in cultural heritage items, identifying the main biological agents responsible for the damage using molecular approaches. OF 11) Integrate theoretical and practical knowledge to propose scientifically grounded conservation strategies. OF 12) Be able to scientifically assess the level of biological deterioration in cultural heritage, considering the type of material and its conservation environment. OF 13) Develop the ability to design and plan a laboratory dedicated to ancient DNA analysis, taking into account technical, logistical, and biosafety considerations. D - Communication skills OF 14) Be able to effectively communicate the results of biological analyses applied to cultural heritage, using terminology appropriate to scientific and multidisciplinary contexts. OF 15) Develop oral and written presentation skills in English, to convey findings to diverse audiences, such as specialists, conservators, and non-academic stakeholders. E - Ability to learn OF 16) Develop the ability to stay updated in the field of biological methods applied to cultural heritage, including through the use of international scientific databases such as GenBank and BOLD. OF 17) Acquire project and critical thinking skills to design and organize research activities, such as the setup of a laboratory for DNA analysis. OF 18) Strengthen the capacity for interdisciplinary learning for effectively addressing the evolving technologies and challenges in cultural heritage conservation.

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LAURA PARDUCCI Lecturers' profile

Program - Frequency - Exams

Course program
The course consists of two modules of three ECTS each. The first module focuses on advanced methodologies for the study of biodeterioration and the reconstruction of past environments. This includes traditional proxies for the investigation of animal and plant fossil remains from archaeological contexts and the utilization of more advanced DNA-based methodologies. The second module concentrates on the application of DNA analysis to cultural heritage, with a specific emphasis on the conservation of artworks. Topics to be discussed include genetic diversity and evolution, tools to identify living organisms, genetic markers, phylogenetic inference, and main DNA-based laboratory techniques such as DNA extraction, amplification of DNA fragments via PCR, DNA-fingerprinting, and sequencing. Practical activities include laboratory work, SEM and 3D digital microscopy, and the writing of scientific notes.
Prerequisites
A good knowledge of English is necessary. A basic school knowledge of biology and genetics is also desirable.
Books
Material available during the course on the e-learning Moodle platform.
Teaching mode
The lessons provide an overview of the main topics. Practical activities include, laboratory work, SEM and 3D digital microscopy, writing scientific notes.
Frequency
The lessons provide an overview of the main topics. Attendance at lessons is not compulsory but it is desirable: frequent absences result in a reduced ability to answer the exam questions.
Exam mode
An intermediate project with a short (15 min) oral presentation of a selected topic (25%) and a final oral examination (75%) verify the student's understanding of the course topics.
Lesson mode
6 credits Lectures approximately 32 hours, 16-18 two-hour lectures and practical exercises to be carried out in class and in the laboratory, approximately 24 hours
  • Academic year2025/2026
  • CourseScience and Technology for the Conservation of Cultural Heritage
  • CurriculumScience and Technology for the Conservation of Cultural Heritage - in lingua inglese
  • Year1st year
  • Semester2nd semester
  • SSDBIO/07
  • CFU3