MARINA BOUCHE'

Marina Bouché: CURRICULUM VITAE
Nationality Italian
Education Degree in Biology, with honors, Sapienza Univ. of Rome, 1979
Maturità Classica, Liceo T. Mamiani, Rome, 1974
Address Dept. of Anatomy, Histology, Forensic Medicine and Orthopedics, Unit of Histology and Med. Embryology, Sapienza Univ. of Rome, Via A. Scarpa14, 00161 Rome, Italy.
Tel. +39 (6) 4976 6755 / 6573
E-mail : marina.bouche@uniroma1.it

Present Position
Associate Professor in Histology, Medical School, Sapienza Univ. of Rome
She is qualified as Full Professor in Histology

Professional experience
2001-present Associate Professor in Histology, Medical School, Sapienza Univ. of Rome.
2024 Invited Visiting Professor, Laboratory of Clinical Investigation - Experimental Surgery
Hospital das Clinicas, Univ. of Sao Paulo, Brazil
2008 Invited Visiting scientist, Groupe Miologie, UMR S 787 Inserm Université
Paris 6 PMC, Paris.
2000-2001 Research Associate, Dept. of Histology, Sapienza Univ. of Rome
1990-2000 Technologist, Dept. of Histology, Sapienza Univ. of Rome.
1993 Visiting scientist (NCI) Cell Biol. UT Southwestern at Dallas, USA.
1986-1990 Post_doctoral Research Fellow, Inst. of Histology, Sapienza Univ. of Rome.
1984-1986 Post_doctoral Research Fellow, Cell Biology, Cornell Univ. Med. College,
New York, USA;
1982-1984 Post_doctoral Research Fellow, Inst. of Histology, Sapienza Univ. of Rome.
1979-1982 Instructor, Inst. of Histology, Sapienza Univ. of Rome.

Fields of interests
Since the beginning Prof. Bouché is being interested on the mechanisms regulating skeletal and cardiac muscle differentiation, histogenesis and remodeling in physiological and pathological conditions. In the last years her activity has been mostly focused on the identification of molecular targets for the therapy of degenerating myopathies, such as Duchenne Muscular Dystrophy. It is becoming clear that a finely regulated crosstalk between muscle cells, interstitial cells and immune cells, which includes endocrine/paracrine signals and cell-to-cell interactions, is required for muscle repair following damage, as well as for maintenance of muscle homeostasis. Alterations in these mechanisms lead to inefficient repair following acute injury or aging or genetic diseases, such as DMD.
Aim of Prof. Bouché research is to identify molecular targets to qualitatively modulate immune response in myopathies, in particular in DMD, to modify the microenvironment and favor tissue repair in replacing muscle loss.

Publications
More than 60 papers published in indexed peer-reviewed international journals, among which more than the half as first or last author; No. 3 papers published in peer-reviewed international journals (not-indexed); No. 2 papers published in monographs.
Total IF (JCR 2020) ~ 400 (Average IF ~5)
H Index: 26
Total Citations: ~6000
More than 200 Congress Communications, many of which published in Abstract Issues of indexed peer review international journal
She is contributing author of the Embryology textbook for the medical students “EMBRIOLOGIA UMANA: morfogenesi, processi molecolari, aspetti clinici” (De Felici et al., Piccin I Ed., 2009, II Ed. 2014, III Ed. 2020).
She is contributing author of the Histology textbook for the medical students “ISTOLOGIA di Monesi” (Adamo et al., Piccin 7°Ed. 2018), as well as of the textbook “Elementi di Istologia e cenni di Embriologia” for the health professional courses and biotechnology (a cura di A. Filippini, Piccin I Ed, 2020)

Peer Review and Scientific Societies
Member of the Editorial Board of International Journal of Molecular Science
Member of the Editorial Board of Frontiers in Physiology
Member of the Editorial Board of European Journal of Translational Myology
Member of the Editorial Board of The Scientific World Journal
2021 she served as Lead Guest Editor for the Special Issue entitled “Signaling Pathways in Striated Muscle Differentiation, Histogenesis and Repair” for the journal International Journal of Medical Sciences.
2019 she served as Lead Guest Editor for the Special Issue entitled “Signaling Pathways in Skeletal Muscle Differentiation, Histogenesis and Repair” for the journal International Journal of Medical Sciences.
2014 – she served as Lead Guest Editor for the Special Issue entitled “Inflammation in Muscle Repair,
Aging, and Myopathies” for the journal Biomedical Research International.
2001-present – she serves as reviewer for many journals and agencies, including: Immun Lett, Stem
Cells, FASEB J, Febs J, Oncogene, Frontiers in Physiology, Exp. Cell Res., PLoS One, J. Pharm and Pharmacology, Frontiers in Immun., Mol and Cell. Endocr., Cell Proliferation, BMC Cell Biology, J. Appl Physiol, BBA, J. of Pathology, Autophagy, Aging Res. Rev., Annals of Anatomy, MIUR (PRIN/FIRB), AFM (French Association on Myopathies), ANR (French national Agency of Research), Sorbonne University.

Meetings and Workshops
Dr. Bouche has been presenting her work at meetings and workshops organized by many scientific societies
among which: Gordon, EMBO, Biochemical Society, American Society of Cell Biology, American Society
of Biological Chemists, Biophysical Society, American Society of Anatomists, Keystone Symposia, British
Society for Experimental Biology, ABCD, FISV.

Awards and Grants
During the years her research have been and is supported by funds obtained from several agencies including:
MIUR-PRIN, Sapienza Univ. of Rome, Telethon Italy, French Association on Myopathies (AFM), Dutch
Parent project NL (DPP), Parent Project Italy, NIH/CNR exchange grants.
Teaching experience
Since 2001 teaches Histology at the Dental School, Sapienza Univ. of Rome.
Since 2004 teaches Cell Biotechnology at the Medical Biotechnology School, Sapienza Univ. of Rome
Since 2019 teaches Histology at the Obstetrics School, Sapienza Univ. of Rome
Since 2019 teaches Histology at the Medical School, Sapienza Univ. of Rome
During the period 2001-2019 she taught Histology at the Biotechnology School (1 level), as well as at the Practical Nurse School, Sapienza Univ. of Rome
During the period 2011-2017 she taught Histology at the International Medical School, Sapienza Univ. of Rome
During the period 2001-2004 she taught Histology and Embryology at the Obstetrics and Gynaecology
Registered School, Policlinico Umberto I, Rome
In 2000-2001 she taught Histology and Embryology at the Medical School, II University of Naples.
In 1999-2000 contributed to the Histology and Embryology course at the Medical School, II University of
Naples.
During the period 1996-2001 contributed as Instructor (practical lessons, exams) to the teaching of Histology
and Gen Embr. at the Dental School, Sapienza Univ. of Rome
In 1995-96 contributed to the “Canale Didattico Sperimentale” (Canale Parallelo Romano), Sapienza Univ.
of Rome.
From 1980 to 1996 contributed as Instructor (practical lessons, exams) to the teaching of Histology and Gen
Embr. after which to the Histology Course at the Medical school, at the Nurse School as well as at the
Registered School in Medicine, Sapienza Univ. of Rome.
She is member of teacher’s committee of the PhD School in “Morphogenesis and Tissue Engineering”, Sapienza Univ. of Rome
She followed and currently follows, as Tutor, master, under-graduate, and graduate students for the preparation of their theses.
Selected Publications (last 15 years, in reverse chronological order)
1. Gloriani M., Cheli B., D’Ercole C., Ruggieri V., Cosentino M., Serrat Pineda M., Lozanoska-Ochser B., Grassi F., Bouché M., Madaro L. & Sánchez Riera C. Sarcoglycans are enriched at the neuromuscular junction in a nerve-dependent manner. Cell Death & Disease 2025, 16, 37. DOI: 10.1038/s41419-025-07353-1. IF 8,1
2. Millozzi F., Milán-Rois P., Sett A., Delli Carpini G., De Bardi M., Gisbert-Garzarán M., Sandonà M., Rodríguez-Díaz C., Martínez-Mingo M., Pardo I., Esposito F., Viscomi MT., Bouché M., Parolini O., Saccone V., Toulmé J., Somoza A. & Palacios D. Aptamer-conjugated gold nanoparticles enable oligonucleotide delivery into muscle stem cells to promote regeneration of dystrophic muscles. Nature Communications 2025, 16, 577. DOI:10.1038/s41467-024-55223-9. IF 14,7
3. Ruggieri V., Scaricamazza S., Bracaglia A., D’Ercole C., Parisi C., D’Angelo P., Proietti D., Cappelletti C., Macone A., Lozanoska-Ochser B., Bouche M., Latella L., Valle C., Ferri A., Giordani L. and Madaro L. Polyamine metabolism dysregulation contributes to muscle fiber vulnerability in ALS. Cell Reports 2025, 44, 115123. DOI: 10.1016/j.celrep.2024.115123. IF 7,5
4. Morroni J., Benedetti A., Esposito L. De Bardi M., Borsellino G., Sanchez Riera C., Giordani L., Bouché M.* and Lozanoska-Ochser B.* Injury-experienced satellite cells retain long-term enhanced regenerative capacity. Stem Cell Res Ther 2023, 14, 246. DOI: 10.1186/s13287-023-03492-4. IF 7,5 (*co-senior authors)
5. Della Peruta C., Lozanoska-Ochser B., Renzini A., Moresi V., Sanchez Riera C., Bouché M.*, and Coletti D. Sex Differences in Inflammation and Muscle Wasting in Aging and Disease. Int J Mol Sci 2023, 24, 4651. DOI: 10.3390/ijms24054651. IF 5,9 (*corresponding author)
6. Millozzi F., Papait A., Bouché M., Parolini O. and Palacios D. Nano-immunomodulation: a new strategy for skeletal muscle diseases and aging? Int J Mol Sci 2023, 24: 1175. DOI: 10.3390/ijms24021175. IF 5,9
7. Di Rocco A., Camero S., Benedetti A., Lozanoska-Ochser B., Megiorni F., Marchese C., Stramucci L., Ciccarelli C., Bouché M., Bossi G., Marampon F. and Zani BM. Anti‑oncogenic and pro‑myogenic action of the MKK6/p38/AKT axis induced by targeting MEK/ERK in embryonal rhabdomyosarcoma. Oncology Reports 2022, 48: 151. DOI: 10.3892/or.2022.8363. IF 3,9

8. Morroni J., Schirone L., Valenti V., Zwergel C., Sánchez Riera C., Valente S., Vecchio D., Schiavon S., Ragno R., Mai A., Sciarretta S., Lozanoska-Ochser B. and Bouchè M. Inhibition of PKCθ Improves Dystrophic Heart Phenotype and Function in a Novel Model of DMD Cardiomyopathy. Int J Mol Sci 2022, 23: 2256. DOI: 10.3390/ijms23042256. IF 5,9

9. Benedetti A., Cera G., De Meo D., Villani C., Bouche M., and Lozanoska-Ochser B. A Simple Method for the Isolation and in vitro Expansion of Highly Pure Mouse and Human Satellite Cells. Bio-Protocol 2021, 11, e4238. DOI: 10.21769/BioProtoc.4238.

10. Morroni, J.; Schirone, L.; Vecchio, D.; Nicoletti, C.; D’Ambrosio, L.; Valenti, V.; Sciarretta, S.; Lozanoska-Ochser, B.; Bouchè, M. Accelerating the Mdx Heart HistoPathology through Physical Exercise. Life 2021, 11, 706. DOI: 10.3390/life11070706. IF: 3,8

11. Proietti D., Giordani L., De Bardi M., D’Ercole C., Lozanoska-Ochser B., Amadio S., Volonté C., Marinelli S., Muchir A.C., Bouché M., Borsellino G., Sacco A., Puri PL., and Madaro L. Activation of skeletal muscle–resident glial cells upon nerve injury. JCI Insight 2021, 6,143469. DOI: 10.1172/jci.insight.143469. IF: 8,3

12. Sánchez Riera C., Lozanoska-Ochser B., Testa S., Fornetti E., Bouché M. and Madaro L. Muscle Diversity, Heterogeneity, and Gradients: Learningfrom Sarcoglycanopathies. Int J Mol Sci 2021, 22, 2502. DOI: 10.3390/ijms22052502. IF 5,9

13. Benedetti A., Cera G., De Meo D., Villani C., Bouche M.* and Lozanoska-Ochser B.* A novel approach for the isolation and long-term expansion of pure satellite cells based on ice-cold treatment. Skelet Muscle 2021, 11,7. DOI: 10.1186/s13395-021-00261-w. IF 4,9 (*co-senior authors)

14. Berardi E., Madaro L., Lozanoska-Ochser B., Adamo S., Thorrez L., Bouche M.*, and Coletti D. A Pound of Flesh: What Cachexia is and What It is Not. Diagnostics 2021, 11, 116. DOI:10.3390/ diagnostics11010116. IF 3,7 (*corresponding author).

15. Sandonà M., Consalvi S., Tucciarone L., DeBardi M., Scimeca M., Angelini DA., Buffa V., D’Amico A., Bertini E., Cazzaniga S., Bettica P., Bouché M., Bongiovanni A., Puri PL. and Saccone V. HDAC inhibitors tune miRNAs in extracellular vesicles of dystrophic muscle-resident mesenchymal cells. EMBO reports 2020 21: e50863. DOI:10.15252/embr.202050863. IF: 8,8

16. Benedetti A, Fiore PF, Madaro L, Lozanoska-Ochser B and Bouché M. Targeting PKCθPromotes Satellite Cell Self-Renewal. Int J Mol Sci. 2020 Mar 31. pii: E2419. DOI: 10.3390/ijms21072419. IF: 5,9

17. Fiore PF, Benedetti A, Sandonà M, Madaro L, De Bardi M, Saccone V, Puri PL, Gargioli C, Lozanoska-Ochser B and Bouché M. Lack of PKC promotes regenerative ability of muscle stem cells in chronic muscle injury. Int J Mol Sci. 2020 Jan 31. pii: E932. DOI: 10.3390/ijms21030932. Scopus ID: 2-s2.0-85079066078. IF: 5,9

18. Rizzo G, Di Maggio R, Benedetti A, Morroni J, Bouche M*, Lozanoska-Ochser B*. Splenic Ly6Chi monocytes are critical players in dystrophic muscle injury and repair. JCI Insight. 5, pii: 130807, 2020. [Epub ahead of print 2019 Dec 24] DOI: 10.1172/jci.insight.130807. WOS: WOS:000510827200008, Scopus ID: 2-s2.0-85079546863. IF: 8,3 (*co-senior authors)

19. Madaro L, Torcinaro A, De Bardi M, Pelizzola M, Diaferia GR, Imeneo G, Bouchè M, Puri PL, and De Santa F. Macrophages fine tune satellite cell fate in dystrophic skeletal muscle of mdx mice. PLoS Genet. 15: e1008408, 2019. DOI: 10.1371/journal.pgen.1008408; Scopus ID: 2-s2.0-85074307670. IF: 5,9.

20. Adamo S, Pigna E, Lugarà R, Moresi V, Coletti D and Bouché M. Skeletal muscle: a significant novel neurohypophyseal hormone-secreting organ. Front Physiol. 9: 1885, 2019. DOI: 10.3389/fphys.2018.01885; WOS: 000455141800002; Scopus ID: 2-s2.0-85069781280. IF: 4,56
21. Bouchè M, Lozanoska-Ochser B, Proietti D, Madaro L. Do neurogenic and cancer-induced muscle atrophy follow common or divergent paths? Eur J Transl Myol 28: 393-400, 2018. DOI: 10.4081/ejtm.2018.7931; WOS:000455715500009; Scopus ID: 2-s2.0-85059286275. IF: 0,6

22. Ciccarelli C, Di Rocco A, Gravina GL, Mauro A, Festuccia C, Del Fattore A, Antinozzi C, Berardinelli P, De Felice F, Musio D, Bouché M, Crescioli C, Tombolini V, Zani BM, Marampon F. Disruption of MEK/ERK/c-Myc signalling radiosensitizes prostate cancer cells in vitro and in vivo. J. Cancer Res Clin Oncol 2018. Epub 2018 June 29. DOI: 10.1007/s00432-018-2696-3; WOS: 000440283600005; Scopus ID: 2-s2.0-85049135239. IF: 4,5

23. Renzini A, Benedetti A, Bouchè M, Silvestroni L, Adamo S, Moresi V. Culture conditions influence satellite cell activation and survival of single myofibers. Eur J Transl Myol 28: 167-174, 2018. DOI: 10.4081/ejtm.2018.7567; WOS:000455714800003. IF: 0,6

24. Lozanoska-Ochser B, Benedetti A, Rizzo G, Marrocco V, Di Maggio R, Fiore P, Bouché M. Targeting early PKCθ-dependent T cell infiltration of dystrophic muscle reduces disease severity in a mouse model of muscular dystrophy. J. of Pathol 244: 323-333, 2018 (Epub 2018, Jan 9) DOI: 10.1002/path.5016; WOS:000425116400008; Scopus ID 2-s2.0-85040202197. IF: 7,99

25. Dobrowolny G, Martini M, Scicchitano BM, Romanello V, Boncompagni S, Nicoletti C, Pietrangelo L, De Panfilis S, Catizone A, Bouche M, Sandri M, Rudolf R, Protasi F, Musaro M. Muscle expression of SOD1G93A triggers the dismantlement of neuromuscular junction via PKC-theta. Antioxidants & Redox Signaling, 28:1105-1119, 2018 (Epub 2017, Oct 30) DOI: 10.1089/ars.2017.7054; WOS:000414464900001; Scopus ID. 2-s2.0-85041995844. IF: 8,4

26. Marrocco V, Fiore P, Benedetti A, Pisu S, Rizzuto E, Musarò A, Madaro L, Lozanoska-Ochser B, Bouché M. Pharmacological inhibition of PKCθ counteracts muscle disease in a mouse model of Duchenne Muscular Dystrophy. EBioMedicine, 16: 150-161, 2017. DOI: 10.1016/j.ebiom.2017.01.001; WOS:000395262400028; Scopus ID 2-s2.0-85009811544. IF: 8,14

27. Klionsky D.J., K. Abdelmohsen,… J. Botti, G. Bou, M. Bouché, M. Bouchecareilh, M. Boucher,…. T. Zoladek, W. Zong, A. Zorzano, S.M. Zughaier. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy, 12: 1-222, 2016. WOS:000373595400001; Scopus ID 2-s2.0-85013763791. IF: 16

28. Smeriglio P., S. Alonso-Martin, S. Masciarelli, L. Madaro, I. Iosue, V. Marrocco, F. Relaix, F. Fazi, G. Marazzi, D. A. Sassoon, M. Bouché. Phosphotyrosine phosphatase inhibitor bisperoxovanadium endows myogenic cells with enhanced muscle stem cell functions via epigenetic modulation of Sca-1 and Pw1 promoters. Faseb J. 30:1404-1415, 2016; published ahead of print December 15, 2015. WOS:000372629100004; Scopus ID 2-s2.0-84963957215. IF: 5,1

29. Faggi F., N. Chiarelli, M. Colombi, S. Mitola, R. Ronca, L. Madaro, M. Bouche, P.L. Poliani, M. Vezzoli, F. Longhena, E. Monti, B. Salani, D. Maggi, C. Keller, A. Fanzani. Cavin-1 and Caveolin-1 are both required to support cell proliferation, migration and anchorage-independent cell growth in rhabdomyosarcoma. Lab. Invest. 95:585-602, 2015; published ahead of print March 30, 2015. WOS:000355145600002; Scopus ID 2-s2.0-84930021783. IF: 5,66

30. Marrocco V., P. Fiore, L. Madaro, A. Crupi , B. Lozanoska-Ochser, M. Bouché. Targeting PKCθ in skeletal muscle and muscle diseases: good or bad? Biochem Soc Trans. 42:1550-5, 2014. WOS:000345427100013; Scopus ID 2-s2.0-84911908641. IF: 5,4

31. Bouché M., P. Muñoz-Cánoves, F. Rossi, and D. Coletti . Inflammation in Muscle Repair, Aging, and Myopathies. BioMed Res. Int. 2014: 821950. Epub 2014 Aug 4. WOS:000340386400001; Scopus ID 2-s2.0-84926527757. IF: 3,4

32. Madaro L. and M. Bouché. From Innate to Adaptive Immune Response in Muscular Dystrophies and Skeletal Muscle Regeneration: The Role of Lymphocytes. BioMed Res. Int. 2014:438675. Epub 2014 Jun 16. WOS:000338063800001; Scopus ID 2-s2.0-84904214970. IF: 3,4

33. Camerino GM., M. Bouchè, M. De Bellis, M. Cannone, A. Liantonio, K. Musaraj, R. Romano, P. Smeriglio, L. Madaro, A. Giustino, A. De Luca, JF. Desaphy, D. Conte Camerino, S. Pierno. Protein kinase C theta (PKCθ) modulates the ClC-1 chloride channel activity and skeletal muscle phenotype: a biophysical and gene expression study in mouse models lacking the PKCθ. Pflugers Arch - Eur J Physiol 466: 2215-28, 2014. Epub 2014, Mar 20. WOS:000345093400005; Scopus ID 2-s2.0-84912021603. IF: 3,65

34. Madaro L, F. Antonangeli, A. Favia, B. Esposito, F. Biamonte, M. Bouché, E. Ziparo, G. Sica, A. Filippini, A. D'Alessio. Knock down of caveolin-1 affects morphological and functional hallmarks of human endothelial cells. J Cell Biochem. 114: 1843-51, 2013. WOS:000320276000015; Scopus ID 2-s2.0-84879171327. IF: 4,42

35. Madaro L., V. Marrocco, S. Carnio, Sandri M. and M. Bouché. Intracellular signaling in ER stress-induced autophagy in skeletal muscle cells. Faseb J. 27:1990-2000, 2013. [Epub 2013 Feb 6]. WOS:000318226100019; Scopus ID 2-s2.0-84877101291. IF: 5,1

36. Madaro L., A. Pelle, C. Nicoletti, A. Crupi, V. Marrocco, G. Bossi, S. Soddu and M. Bouché. PKC theta ablation improves healing in a mouse model of muscular dystrophy. PLoSOne7: e31515. Epub 2012 Feb 14. WOS:000302737400026; Scopus ID 2-s2.0-84856850591. IF: 3,24

37. Verga Falzacappa C., C. Mangialardo, L. Madaro, S. Raffa, D. Ranieri, A. Stigliano, E. Brunetti, MR. Torrisi, M. Bouchè, V. Toscano and S. Misiti. Thyroid hormone T3 prevents STZ induced diabetes in mouse PLoSOne 6: e19839. Epub 2011 May 27. WOS:000291052500013; Scopus ID 2-s2.0-80053310960. IF: 3,24

38. Quattrocelli M, G Palazzolo, I Agnolin, S Martino, M Bouché, L Anastasia, M. Sampaolesi. Synthetic sulfonyl-hydrazone-1 positively regulates cardiomyogenic microRNA expression and cardiomyocyte differentiation of induced pluripotent stem cells. J. Cell. Biochem, 112: 2006-14, 2011. WOS:000293329300007; Scopus ID 2-s2.0-79960378031 IF: 4,42

39. Madaro L., V. Marrocco, P. Fiore, P.Aulino, P. Smeriglio, S. Adamo, M. Molinaro and M. Bouché. Protein kinase C theta signaling is required for myoblast fusion by regulating the expression of caveolin-3 and β1D integrin upstream Focal Adhesion Kinase. Mol. Biol. Cell, 22: 1409-1419, 2011. [Epub 2011, Feb 23]. WOS:000289558600025; Scopus ID 2-s2.0-79954602263 IF: 4,13

40. Paoletti R., A. Notte, A. Maffei, E. Stanganello, G. Cifelli, G. Selvetella Molinaro M., Lembo G. and Bouché M. Protein kinase C theta is required for cardiomyocytes survival and cardiac remodelling. Cell Death and Disease, 1: e45, Epub 2010 May 27. WOS:000279818500007; Scopus ID 2-s2.0-79954600897 IF: 6,304

Books

o De Felici M, Boitani C, Bouche M, Canipari R, Dolfi A, Filippini a, Musarò A, Papaccio G, Salustri A. Embriologia Umana: morfogenesi, processi molecolari, aspetti clinici, Piccin I Ed., 2009, II Ed. 2014, III Ed. 2020; Textbook in Human Embryology for Medical School and Biomedical technology students.
o AAVV. ISTOLOGIA di Monesi” (Adamo et al., Piccin 7°Ed. 2018); Textbook in Histology for Medical School students.
o AAVV Elementi di Istologia e cenni di Embriologia, (a cura di A. Filippini, Piccin I Ed, 2020); Testbook for the health professional and biotechnology students.

Rome March 31st, 2025