GIANNI PROSSEDA

Le lezioni di microbiologia e virologia del CdL di Sc. Biologiche per l'A.A. 2024-2025 inizieranno il 27 settembre 2024 in aula Sergi (Antropologia) secondo l'orario ufficiale.

Le lezioni di Microbiologia Generale, Biotecnologie Microbie e eìElementi di Microbiologia Medica (II modulo)  CdL di Biotecnologie per l'A.A. 2024-2025 inizieranno il 02 Ottobre  2024 in aula B2 Polo odontoiatrico Via Caserta, secondo l'orario ufficiale.

The course of Microbiology (Bioinformatics course degree, A.A. 2024-2025) will start on 02 October 2024 (16:00-19:00) and will be held on Mondays (16:00-18:00) and Wednesdays (16:00-19:00).

G. Prosseda’s Curriculum Vitae
Positions and Education

• 2022-2031: National Scientific Qualification cat Full Professor (05/I2-Microbiology)
• Since 2019: Associate Professor of Microbiology (SSD/ BIO19), Dept. Biology and Biotechnologies C. Darwin, Faculty of Sciences, Sapienza University of Rome
• 2002-2019: Researcher at the Dept. of Cell and Developmental Biology, Faculty of Sciences, Sapienza University of Rome
• 2001: Advanced School in Biotechnological Application (maximum honors), from the University of Rome Sapienza.
• 2000: Ph.D. in Cellular and Developmental Biology, from the University of Rome "Sapienza.
• 1997: Master in Planning expert of quality assurance: food, packaging and chemical industry from IFOA, Camera del Commercio, Latina.
• 1994: Laurea in Biological Science (maximum honours), from the University of Rome "Sapienza.

Other appointments

Teaching
Since 2018 to 2021 and from 2024: Professor of Microbiology for students in Bioinformatics at Sapienza University of Rome
In 2016: Professor of Applied Microbiology for the student in Sciences and Technologies for the Quality and Enhancement of Agri-food Products at Sapienza University of Rome
Since 2008: Professor of General Microbiology, Microbial Biotechnologies and Element of Medical Microbiology II for the student in Biotechnology at Sapienza University of Rome
From 2003 to 2007 Professor of General Microbiology and Microbial Biotechnologies for the student in Biotechnology at Sapienza University of Rome
September 2019: Invited teacher at the COURS DE MICROBIOLOGIE 2019-2020 at the Institut Pasteur (Paris, France).

Committee:
2025: Member of the Council of the Faculty of Mathematical, Physical, and Natural Sciences, Sapienza Univ. of Rome.
2024: Member of the Council of Department Biology and Biotechnologies Charles Darwin department, Sapienza Univ. of Rome.
2024: Member of the Scientific Technical Committee (STC) Prin 2022 and Prin 2022 PNRR, Dept Biology and Biotechnologies « Charles Darwin »
2024: Member of the Executive Committee of the SIMGBM for the three years 2025-2027, on "Genetics of Microbiology".
2020: Member of the Committee for the Qualifying examinations for Biologist, Sapienza University of Rome
I was president of the Committee for admission to the degree course in Biotechnology at the Sapienza University of Rome in 2011, 2012, 2013, 2014, 2016, and 2018.
In 2015, a member of the Ph.D. Committee in Biology Applied to Human Health (BASU), Department of Sciences, University of Roma Tre
In 2014 Member of the Ph.D. Committee in Cellular and Molecular Biology at the Alma Mater Studiorum - University of Bologna
2011 Member of the Ph.D. Committee in Biology (XXIV Cycle), Roma Tre University.
2009: Member of the Ph.D. Committee in Public Health Sciences and Microbiology (XXI Cycle), Sapienza University of Rome Tre.
2008: Member of the Committee for a research position at the Faculty of Sciences M.F.N. of the University of Salento.

Editorial activity
Since 2018: Member of the editorial board of Frontiers in Cellular and Infection Microbiology
Since 2018: Member of the editorial board of Scientific Report
2003 and 2007: English to Italian translator of the 10th, 11th and 13th editions of the textbook Brock: Biology of Microorganisms Prentice Hall.

Manuscript reviewing/referee activity
Plos ONE, Frontiers in Microbiology, FEMS Letters, Genes, BMC Microbiology, Frontiers in Cellular and Infection Microbiology, APMIS, OBMN Genetics, International Journal of Medical Microbiology, etc. .

Publications over the last 10 years
• Polani R, De Francesco A, Tomolillo D, Artuso I, Equestre M, Trirocco R, Arcari G, Antonelli G, Villa L, Prosseda G, Visca P, Carattoli A. Cefiderocol Resistance Conferred by Plasmid-Located Ferric Citrate Transport System in KPC-Producing Klebsiella pneumoniae. Emerg Infect Dis. 2025 Jan;31(1):123-124. doi: 10.3201/eid3101.241426. PMID: 39714320. IF2023: 7.2
• Ishikawa T, Eguchi Y, Igarashi M, Okajima T, Mita K, Yamasaki Y, Sumikura K, Okumura T, Tabuchi Y, Hayashi C, Pasqua M, Coluccia M, Prosseda G, Colonna B, Kohayakawa C, Tani A, Haruta JI, Utsumi R. Synthesis and biochemical characterization of naphthoquinone derivatives targeting bacterial histidine kinases. J Antibiot (Tokyo). 2024 Jun 25. doi: 10.1038/s41429-024-00726-2. Epub ahead of print. PMID: 38918599. IF2022: 3.3
• Rosignoli S, Lustrino E, Conci A, Fabrizi A, Rinaldo S, Latella MC, Enzo E, Prosseda G, De Rosa L, De Luca M, Paiardini A. AlPaCas: allele-specific CRISPR gene editing through a protospacer-adjacent-motif (PAM) approach. Nucleic Acids Res. 2024 May 25: gkae419. doi: 10.1093/nar/gkae419. Epub ahead of print. PMID: 38795068. IF2022: 14.9
• Trirocco R, Pasqua M, Tramonti A, Colonna B, Paiardini A, Prosseda G. Diffusible signal factors (DSFs) bind and repress VirF, the leading virulence activator of Shigella flexneri. Sci Rep. 2023 Aug 14;13(1):13170. doi: 10.1038/s41598-023-40023-w. PMID: 37580399.
IF2023: 4.997.
• Coluccia M, Béranger A, Trirocco R, Fanelli G, Zanzi F, Colonna B, Grossi M, Prosseda G, Pasqua M. Role of the MDR Efflux Pump AcrAB in Epithelial Cell Invasion by Shigella flexneri. Biomolecules. 2023 May 11;13(5):823. doi: 10.3390/biom13050823. PMID: 37238693. IF2023: 5.5
• Carev I, Gelemanović A, Glumac M, Tutek K, Dželalija M, Paiardini A, Prosseda G. Centaurea triumfetii essential oil chemical composition, comparative analysis, and antimicrobial activity of selected compounds. Sci Rep. 2023 May 8;13(1):7475. doi: 10.1038/s41598-023-34058-2. PMID: 37156785. IF2023: 4.997.
• Trirocco R, Pasqua M, Tramonti A, Grossi M, Colonna B, Paiardini A, Prosseda G. Fatty Acids Abolish Shigella Virulence by Inhibiting Its Master Regulator, VirF. Microbiol Spectr. 2023 Jun 15;11(3):e0077823. doi: 10.1128/spectrum.00778-23. Epub 2023 May 4. PMID: 37140433. IF2023: 3.7
• Fanelli G, Pasqua M, Prosseda G, Grossi M, Colonna B. AcrAB efflux pump impacts on the survival of adherent-invasive Escherichia coli strain LF82 inside macrophages. Sci Rep. 2023 Feb 15;13(1):2692. doi: 10.1038/s41598-023-29817-0. PMID: 36792672. IF2023: 4.997
• Pasqua M, Coluccia M, Eguchi Y, Okajima T, Grossi M, Prosseda G, Utsumi R, Colonna B. Roles of Two-Component Signal Transduction Systems in Shigella Virulence. Biomolecules. 2022 Sep 18;12(9):1321. doi: 10.3390/biom12091321. PMID: 36139160. IF2022: 5.5
• Skovajsová E, Colonna B, Prosseda G, Sellin ME, Di Martino ML. The VirF21:VirF30 protein ratio is affected by temperature and impacts Shigella flexneri host cell invasion. FEMS Microbiol Lett. 2022 Jun 22;369(1):fnac043. doi: 10.1093/femsle/fnac043. PMID: 35521699. IF2022: 2.1
• Pasqua M, Bonaccorsi di Patti MC, Fanelli G, Utsumi R, Eguchi Y, Trirocco R, Prosseda G, Grossi M, Colonna B. Host - Bacterial Pathogen Communication: The Wily Role of the Multidrug Efflux Pumps of the MFS Family. Front Mol Biosci. 2021 Jul 26;8:723274. doi: 10.3389/fmolb.2021.723274. eCollection 2021. PMID: 34381818. IF2021: 5.838
• Pasqua M, Zennaro A, Trirocco R, Fanelli G, Micheli G, Grossi M, Colonna B, Prosseda G. Modulation of OMV Production by the Lysis Module of the DLP12 Defective Prophage of Escherichia coli K12. Microorganisms. 2021 Feb 12;9(2):369. Doi:10.3390/microorganisms9020369. PMID: 33673345. IF2021: 4.926
• Fanelli G, Pasqua M, Colonna B, Prosseda G, Grossi M. Expression Profile of Multidrug Resistance Efflux Pumps During Intracellular Life of Adherent-Invasive Escherichia coli Strain LF82. Front Microbiol. 2020 Aug 17;11:1935. doi: 10.3389/fmicb.2020.01935. eCollection 2020. PMID: 33013734. IF2020: 5.64
• Michelacci V, Tozzoli R, Arancia S, D'Angelo A, Boni A, Knijn A, Prosseda G, Greig DR, Jenkins C, Camou T, Sirok A, Navarro A, Schelotto F, Varela G, Morabito S. Tracing Back the Evolutionary Route of Enteroinvasive Escherichia coli (EIEC) and Shigella Through the Example of the Highly Pathogenic O96:H19 EIEC Clone. Front Cell Infect Microbiol. 2020 Jun 3;10:260. doi: 10.3389/fcimb.2020.00260. eCollection 2020. PMID: 32582565. IF2020: 5.293
• Pasqua M, Grossi M, Zennaro A, Fanelli G, Micheli G, Barras F, Colonna B, Prosseda G. The Varied Role of Efflux Pumps of the MFS Family in the Interplay of Bacteria with Animal and Plant Cells. Microorganisms. 2019 Aug 22;7(9):285. doi: 10.3390/microorganisms7090285. PMID: 31443538. IF2019: 4.152
• Pasqua M, Grossi M, Scinicariello S, Aussel L, Barras F, Colonna B, Prosseda G. The MFS efflux pump EmrKY contributes to the survival of Shigella within macrophages. Sci Rep. 2019 Feb 27;9(1):2906. doi: 10.1038/s41598-019-39749-3. PMID: 30814604. IF2019: 3.998
• Pasqua M, Michelacci V, Di Martino ML, Tozzoli R, Grossi M, Colonna B, Morabito S, Prosseda G. The Intriguing Evolutionary Journey of Enteroinvasive E. coli (EIEC) toward Pathogenicity. Front Microbiol. 2017 Dec 5;8:2390. doi: 10.3389/fmicb.2017.02390. eCollection 2017. PMID: 29259590. IF2017: 4.019
• Leuzzi A, Grossi M, Di Martino ML, Pasqua M, Micheli G, Colonna B, Prosseda G. Role of the SRRz/Rz1 lambdoid lysis cassette in the pathoadaptive evolution of Shigella. Int J Med Microbiol. 2017 Jun;307(4-5):268-275. doi: 10.1016/j.ijmm.2017.03.002. Epub 2017 Mar 28. PMID: 28389211. IF2017: 3.298
• Di Martino ML, Romilly C, Wagner EG, Colonna B, Prosseda G. One Gene and Two Proteins: a Leaderless mRNA Supports the Translation of a Shorter Form of the Shigella VirF Regulator. mBio. 2016 Nov 8;7(6):e01860-16. doi: 10.1128/mBio.01860-16. PMID: 27834204.
IF2016: 6.956
• Di Martino ML, Falconi M, Micheli G, Colonna B, Prosseda G. The Multifaceted Activity of the VirF Regulatory Protein in the Shigella Lifestyle. Front Mol Biosci. 2016 Sep 29;3:61. doi: 10.3389/fmolb.2016.00061. eCollection 2016. PMID: 27747215 Free PMC article. IF2016: 3.276
• Michelacci V, Prosseda G, Maugliani A, Tozzoli R, Sanchez S, Herrera-León S, Dallman T, Jenkins C, Caprioli A, Morabito S. Characterization of an emergent clone of enteroinvasive Escherichia coli circulating in Europe. Clin Microbiol Infect. 2016 Mar;22(3):287.e11-9. doi: 10.1016/j.cmi.2015.10.025. Epub 2015 Nov 10. PMID: 26551840. IF2015: 4.575
• Leuzzi A, Di Martino ML, Campilongo R, Falconi M, Barbagallo M, Marcocci L, Pietrangeli P, Casalino M, Grossi M, Micheli G, Colonna B, Prosseda G. Multifactor Regulation of the MdtJI Polyamine Transporter in Shigella. PLoS One. 2015 Aug 27;10(8):e0136744. doi: 10.1371/journal.pone.0136744. eCollection 2015. PMID: 26313003. IF2015: 3.057
• Campilongo R, Di Martino ML, Marcocci L, Pietrangeli P, Leuzzi A, Grossi M, Casalino M, Nicoletti M, Micheli G, Colonna B, Prosseda G. Molecular and functional profiling of the polyamine content in enteroinvasive E. coli : looking into the gap between commensal E. coli and harmful Shigella. PLoS One. 2014 Sep 5;9(9):e106589. doi: 10.1371/journal.pone.0106589. eCollection 2014. PMID: 25192335. IF2014: 3.234
• Scribano D, Petrucca A, Pompili M, Ambrosi C, Bruni E, Zagaglia C, Prosseda G, Nencioni L, Casalino M, Polticelli F, Nicoletti M. Polar localization of PhoN2, a periplasmic virulence-associated factor of Shigella flexneri, is required for proper IcsA exposition at the old bacterial pole. PLoS One. 2014 Feb 27;9(2):e90230. doi: 10.1371/journal.pone.0090230. eCollection 2014. PMID: 24587292. IF2014: 3.234

Current other Financial Support:
• PI unit 3, PTR 619-23 project: “ShigeFeS”. Title: “Iron-Sulfur Clusters Biogenesis Polymorphism and Virulence in Shigella”; 01/01/2024 - 31/12/2025 (75k)
• PI Sapienza’s Team (partnership with Università di Modena) for the ERC Advanced Grant Holo-GT (PI Prof. De Luca). (2021-2025) (219k)