GENERAL MICROBIOLOGY, MICROBIAL BIOTECHNOLOGIES AND ELEMENTS OF MEDICAL MICROBIOLOGY

Course objectives

General skills Today’s view of Microbiology has gone far beyond the simple idea of microbes as causative agents of a diversified spectrum of human pathologies. The current opinion considers microorganisms mainly as major constituents of ecosystems, vital components of eukaryotic organisms, very often key mediators in productive and industrial processes. Our course in Microbiology is therefore intended to provide students with the knowledge clues required to understand the high plasticity of the microbial world. The focus is on progressively integrating specific notions about bacterial and viral structures, their functional relationships, and the organization and evolution of genomes into a logical learning path. This will secure the understanding of the molecular mechanisms which regulate the interaction of microorganisms with both biotic and abiotic environments. Students will thus acquire the competences to assess the role of microorganisms in biomedical, biotechnological, and environmental area. Specific skills A) Knowledge and understanding. The student will have acquired knowledge and understanding - Structure of the prokaryotic cell (Bacteria and Archea) and biosynthesis of the cell components. - Organization of the bacterial genome and role of the mobile genetic elements - Procedures for microbial growth and for microbial growth control - Processes of bacterial colonization of the host and of the environment - Mechanisms of action of antibiotics and emergence of antibiotic resistance B) Applying knowledge and understanding - Understanding the key role played by microorganisms in the interactions with the host and the environment. - Understanding the biological processes of multicellular organisms banking on the knowledge acquired from the microorganisms -Understanding biotechnological applications of microorganisms C) Making judgments - Being able to analyze in a thoughtful and informed manner the information on the microbes world - Making informed decisions on the use of products derived from bacteria or viruses and on the importance of vaccinations D) Communication skills - Being able to communicate to the public the notions learned on the microbe world in a correct and informed manner using appropriate terminology - Being able to fully interpret any microorganisms-associated phenomena from both a personal and social point of view. E) Learning skills -be able to make logical connection among different topics covered -learning the specific terminology

Channel 1
FIORENTINA ASCENZIONI Lecturers' profile

Program - Frequency - Exams

Course program
Theoretical Lectures (40 hours) Structure and function of the prokaryotic cell (20 hours). The historical roots of microbiology. Physiological diversity of microrganisms. Structure, function and biosynthesis of the cell wall in Bacteria and Archea. Secretion and exportation in Bacteria. Function and structure of the cytoplasmic membrane. Biosynthesis and function of cell surface structures. Bacterial cell division and sporulation. Microbial nutrition and culture media. Regulation of bacterial gene expression. Bacterial genome, plasticity and evolution (10 hours) Organization of bacterial chromosome: the nucleoid. Structure and function of plasmids. The main features of transposable elements and integrons. The role of mobile genetic elements in the horizontal gene transfer. Bacterial conjugation and transformation. Interaction of the microrganisms with biotic and abiotic environments (10 hours) Overview of human microbiome and microbiota. Mechanisms of actions of toxins. Virulence factors and host defence mechanisms. Quorum sensing and biofilms. Mechanisms of action of antibiotics and antibiotic resistance. Signal transductions: from porin biosynthesis to chemiotaxis. Beneficial and harmful microbial interactions with eukaryotic hosts. Bacterial adaptation to the host response. Principles of innate immunity. Laboratory session (12 hours) Preparation of Bacterial culture media. Measurement of bacterial growth: dilutions and viable counts. The Gram stain procedure and the microscopic observation of bacterial cells. Antibiotic susceptibility tests.
Prerequisites
The Course of Microbiology takes place at the second year in the first semester. For the full understanding of the topics of the Course basic knowledge of Biochemistry, Molecular Biology and a sound knowledge of Cellular Biology, is necessary
Books
Textbook Brock Biologia dei Microrganismi. Quattordicesima Edizione 2016 Pearson Ed. Dehò G., Galli E. Biologia dei Microrganismi. 2014. Casa Editrice Ambrosiana (CEA)
Teaching mode
The course is structured in lectures and didactic laboratories. In particular, 40 hours of frontal teaching (5 CFU) and 12 hours of didactic laboratories (1 CFU) are provided. Lessons are held bi-weekly in the classroom. The hours of didactic laboratories are carried out in groups of 20 people (with specific shifts) and take place in laboratories equipped for teaching Microbiology.
Frequency
Attendance of the lessons is not compulsory but strongly recommended.
Exam mode
The examination aims to verify the level of knowledge of the topics and as well as the reasoning skills acquired by the student. The evaluation is expressed in thirtieths (minimum score 18/30, maximum score 30/30 with honors) and corresponds to the weighted average of the scores obtained in the two parts of the Course. The evaluation consists of an written test after which the oral exam is accessed. The examination is carried out taking into consideration the different topics covered during the course and allows to verify the achievement of the objectives in terms of knowledge and skills acquired, to evaluate, the language property and the critical capacity of the student. To pass the examination it is necessary to obtain at least the score of 18/30. To achieve the score of 30/30 cum laude, the student must demonstrate to have acquired an excellent knowledge of the topics covered in the two parts of the Course and to be able to create logical and coherent connections between the various topics studied.
Bibliography
Reference literature is indicated in the textbook.
Lesson mode
The course is structured in lectures and didactic laboratories. In particular, 40 hours of frontal teaching (5 CFU) and 12 hours of didactic laboratories (1 CFU) are provided. Lessons are held bi-weekly in the classroom. The hours of didactic laboratories are carried out in groups of 20 people (with specific shifts) and take place in laboratories equipped for teaching Microbiology.
  • Lesson code1034848
  • Academic year2024/2025
  • CourseFood and Industrial Biotechnology
  • CurriculumSingle curriculum
  • Year2nd year
  • Semester1st semester
  • SSDBIO/19
  • CFU6
  • Subject areaDiscipline biologiche